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Testosterone by LC-MS/MS

Alternate test name

Lab area
Clinical Biochemistry - Special
Method and equipment
Expected turn-around time
14 days
Specimen type

500 uL Serum or Plasma

Specimen requirements
  • Collect 1mL whole blood, spin, separate and freeze (-20oC)
  • Specimens collected on an SST tube should be separated within 90 minutes of receipt.
  • Avoid repeated freeze-thaw cycles.
Storage and transportation


Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance

Testosterone is produced by the adrenals, the theca cells in the ovary and the leydig cells in the testes. As much as 97% of circulating testosterone is bound to serum proteins such as sex hormone binding globulin (SHBG). In the male, testosterone stimulates the maturation of genitalia and secondary sexual characteristics and its measurement is used to investigate sexual dysfunction in juveniles and adults. In females testosterone concentrations are much lower and an elevated concentration may indicate polycystic ovarian syndrome among other conditions. Clinical symptoms of testosterone excess in females include infertility, amenorrhea, obesity and hirsutism.

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