Fluorescence in situ Hybridization (FISH) - Bone Marrow Aspirate/Unstimulated Peripheral Blood

Alternate test name

Bone marrow FISH tests (testing will be based on indication)

Pediatric ALL

B-ALL FISH panel
B-ALL Ph-like ABL class FISH probes (ABL1, ABL2, PDGFRB)
Enumeration probes

o Chromosomes 4, 10

TEL-AML1 (ETV6-RUNX1)
BCR-ABL1
KMT2A (MLL)
TCF3 (E2A)
CDKN2A (P16)
IGH
CRLF2
ZNF384
TCRa/d
ABL1
ABL2
PDGRFB

Pediatric AML

RUNX1T1-RUNX1 (AML1-ETO)
PML-RARA
CBFB-MYH11
KMT2A (MLL)
NUP98
Enumeration probes

o Chromosomes 5 and 7

Other Hematological

PDGFRA
Enumeration probes

o Chromosomes 7 and 8

Lab area

Genome Diagnostics - Cytogenetics

Method and equipment

Direct preparation and unstimulated 24 hour culture; FISH (Fluorescence in situ hybridization)

Expected turn-around time

2-5 days

Specimen type

Bone Marrow Aspirate;
or Peripheral Blood

Specimen requirements

1.5 mL minimum; 2-3 mL if possible

Storage and transportation

Maintain at room temperature. Do not refrigerate or freeze. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.

Special requirements

Collect sample in sodium heparin (rinse syringe with 100 U.S.P. unit/mL of preservative-free Sodium Heparin)

For aspirate: relocate needle prior to sampling if possible.

If the bone marrow aspirate sample is from a new diagnosis leukemia patient, and is less than the recommended volume, submit peripheral blood in sodium heparin in addition to bone marrow aspirate.

Shipping information

The Hospital for Sick Children

Division of Genome Diagnostics

555 University Avenue, Black Wing, Room 3416

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-7200 ext. 2

Hours: Monday to Friday, 8 a.m. to 4:30 p.m.

Off hours: Please send to Rapid Response Laboratory, 555 University Avenue, Room 3642

Email Molecular Lab: molecular.lab@sickkids.ca

Email Cytogenetics: cytogenetics.requests@sickkids.ca