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25-Hydroxyvitamin D

Alternate test name

Vitamin D, 25OH VitD

Lab area
Clinical Biochemistry - General
Method and equipment
Method:    Chemiluminescent Immunoassay (CLIA)
Equipment: Diasorin Liaison XL
Expected turn-around time
7 days
Specimen type

Serum only

Specimen requirements

250 uL

Storage and transportation

Shipped frozen. Avoid repeated freeze-thaw cycles

Stability: 2 days at 2-8oC, 2 months at -20oC


Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Background and clinical significance

Vitamin D is a fat-soluble steroid prohormone mainly produced photochemically in the skin from 7-dehydrocholesterol. Two forms of vitamin D are biologically relevant – vitamin D3 (Cholecalciferol) and vitamin D2 (Ergocalciferol). Epidemiological studies have shown a high global prevalence of vitamin D insufficiency and deficiency. The measurement of vitamin D status provides opportunities for preventive and therapeutic interventions. Vitamin D deficiency is a cause of secondary hyperparathyroidism and diseases resulting in impaired bone metabolism (like rickets, osteoporosis, osteomalacia). Reduced 25-OH vitamin D concentrations in blood (vitamin D insufficiency) have been associated with an increasing risk of many chronic diseases, including common cancers, autoimmune or infectious diseases or cardiovascular problems.

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