Karyotype, GTG-banding -Tissue

Alternate test name

Chromosome analysis

G-banding

Gene name / Alternate gene name

All chromosomes

Lab area

Genome Diagnostics - Cytogenetics

Method and equipment

Cell culture; G-banding

Expected turn-around time

4 weeks

Specimen type

Tissue

Specimen requirements

Minimum 2-3 mm³ fresh tissue (skin biopsy)

Storage and transportation

Refrigerate until time of shipment. Ship sample overnight at room temperature for receipt at SickKids Cytogenetics Laboratory within 24 hours of collection.

Special requirements

Place in sterile container with SickKids Cytogenetics transport media or other sterile culture media.

Shipping information

The Hospital for Sick Children

Division of Genome Diagnostics

555 University Avenue, Black Wing, Room 3416

Toronto, ON

Canada

M5G 1X8

Phone: 416-813-7200 ext. 2

Hours: Monday to Friday, 8 a.m. to 4:30 p.m.

Off hours: Please send to Rapid Response Laboratory, 555 University Avenue, Room 3642

Email Molecular Lab: molecular.lab@sickkids.ca

Email Cytogenetics: cytogenetics.requests@sickkids.ca

Requisition

Karyotype, GTG-banding -Tissue - requisition

Background and clinical significance

Karyotyping of fibroblasts derived from tissue biopsy is usually performed when mosaicism is suspected in a patient, or when results cannot be obtained from peripheral blood.

Disease condition

Constitutional chromosome disorders, including aneuploidy, chromosome rearrangements, and mosaicism