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Dinitrophenylhydrazine, urine

Alternate test name
  • Dinitrophenylhydrazine (DNPH) 
  • 2,4-dinitrophenylhydrazine
Lab area
Clinical Biochemistry - Metabolic Diseases
Method and equipment

The DNPH test uses the 2,4-dinitrophenylhydrazine reagent with which alpha-keto acids and a variety of carbonyl compounds will react and form relatively insoluble crystalling hydrazones (a positive reaction). 

Expected turn-around time
10 days
Specimen type

Fresh random urine 

Specimen requirements

3 mL

Storage and transportation
  • Store at 4oC. Keep cool during transport.
Special requirements

Unacceptable Specimens: Any sample from a patient who has received a radio opaque dye in previous 24 hours, Arginine Tolerance Test and urine contaminated with stool.

Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
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