Cortisol by LC-MS/MS
500 uL Serum or Plasma
- Collect 1mL whole blood, spin, separate and freeze (-20oC)
- Specimens collected on an SST tube should be separated within 90 minutes of receipt.
- Avoid repeated freeze-thaw cycles.
Cortisol measurements are used as a direct monitor of adrenal status and an indirect measure of pituitary hyper or hypofunction. Elevated cortisol levels are associated with adrenal tumors, pituitary tumors or ectopic ACTH-producing tumors. Subnormal cortisol concentrations may indicate generalized adrenal hypofunction or a defect in the metabolic pathway for cortisol biosynthesis.
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