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SickKids

CD163

Lab area
Clinical Biochemistry - Immunology
Method and equipment

Method:  Automated fluorescence immunoassay

Equipment:  Proteinsimple ELLA

Expected turn-around time
14 days (testing completed weekly)
Specimen type

EDTA plasma

Specimen requirements

2 aliquots (each containing 0.3 mL of EDTA plasma)

Storage and transportation
  • Store and transport frozen.
  • Avoid freeze/thaw cycles.
Special requirements
  • Centrifuge for 15 minutes at 1000 RCF within 15 minutes of collection
  • Immediately separate and freeze.
  • If ordering multiple cytokines at a time, minimum volume required is as follows:
    • any combination of: TNF-alpha, CXCL9/MIG1, IFN-gamma, CD163 ==> 2 tubes of 0.3 mL each
    • any combination of: IL-10, IL-18, IL-1beta and IL-6 ==> 2 tubes of 0.3 mL each
Shipping information
The Hospital for Sick Children
Rapid Response Laboratory
555 University Avenue, Room 3642
Toronto, ON
Canada
M5G 1X8
Phone: 416-813-7200
Toll Free: 1-855-381-3212
Hours: 7 days/week, 24 hours/day
Requisition
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